Effect of PGPR Inoculation on Seed Germination and Some Vigor Traits of Asclepias Procera

Document Type : Research Paper

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This experiment was conducted aiming to improve the germination and vigour traits of Asclepias procera by inoculation with PGPR. The study had 8 levels of inoculation including control, Pseudomonas, Azospirillum, Azotobacter, Azospirillum + Pseudomonas, Pseudomonas + Azotobacter, Azospirillum + Azotobacter, Azospirillum + Azotobacter and Pseudomonas in a completely randomized design with four replications. Results showed that inoculation of Calotrope seeds were significantly affected germination traits. The highest germination percentage (72 %) and germination rate (1.8 per day) was observed by inoculation with Pseudomonas putida strain 169 that respectively was 1.8 and 1.63 fold more than control treatment. The greatest root length ( 19.84 mm) and shortest mean germination time ( 7.97 days ) was belonging to the Pseudomonas + Azotobacter inoculation, that respectively was  1.6 and 7 fold more and less than control. Greatest seed vigor index (17.8) was shown by Azotobacter +Azosprillum that were 1.82 fold more than control. This research revealed that the inoculation of rhizobacterial microorganisms is a new approach that can be effective to improve seed germination in Calotrope shrubs.

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