Effect of Simultaneous Application of Pseudomonas fluorescens and Phosphate Fertilizers on Yield and Uptake of Phosphorous and Micronutrients in Canola

Document Type : Research Paper

Authors

1 PhD student of Soil Science Department, Vali-e-Asr University, Rafsanjan

2 Prof. Soil Science Department, Tarbiat Modares University, Tehran

3 Assistant Prof. Soil and Water Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Researcher, Soil and Water Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Plant growth promoting rhizobacteria (PGPR) have a positive effect on crop yield through increasing the availability of phosphorous and other nutrients. The aim of this study was to investigate the effect of simultaneous application of Pseudomonas fluorescens and phosphate fertilizers on yield and nutrient uptake of canola grain. Experiment was carried out in a greenhouseconditions in acompletely randomized design. The treatments were as control, PGPR, single super phosphate (SSP), triple super phosphate (TSP), ammonium phosphate (AP), rock phosphate (RP), single super phosphate+PGPR, triple super phosphate+PGPR, ammonium phosphate+PGPR and rock phosphate+PGPR. The results demonstrated that grain and shoot yield of canola inoculated with PGPR increased by 29.80 and 30.56 percent compared to control treatment, respectively. Simultaneousapplication ofPGPR and phosphate fertilizers was the most effective treatment on grain and shoot yield. The highest amount of grain and shoot yields were obtained from triple super phosphate+PGPR treatment, which were 10.22 and 29.99 g. pot-1, respectively. There was a significant difference among other treatments. When canola plant inoculated with PGPR  Fe, Zn, Mn and Cu uptake increased in canola grain compared to non-inoculated treatments. The highest uptake of Fe, Zn, Mn and Cu were 63.97, 0.76, 0.43 and 0.03 mg pot-1, respectively. There was no significant difference between triple super phosphate+PGPR and ammonium phosphate+PGPR treatments in uptake of Fe, Zn, Mn and Cu.Overall, the combined use of PGPR and P-fertilizers had a positive and significant effect on yield and nutrients uptake of canola. According to the results, the use of PGPR can increase the availability and efficiency of P-fertilizers.

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