Antibiotic and metal resistance of Azotobacteria, Enterobacteria and Pseudomonads isolated from agricultural, pasture and mining soils in Hamedan, Iran

Document Type : Research Paper

Authors

1 PhD Student, Faculty of Agriculture, Department of Soil Science, Bu-Ali Sina University

2 Professor, Faculty of Agriculture, Department of Soil Science, Bu-Ali Sina University, Hamedan, Iran

Abstract

Heavy metals may increase antibiotic resistance in bacteria. The aim of this study was to determine antibiotic-resistance of frequent Gram-negative isolates in agricultural, pasture and mine soils. Hence, 97 isolates from soil samples were selected and antibiotic (7 antibiotics) and heavy metal resistance were determined by disc diffusion and MIC methods. Based on the results, 50% of the Azotobactersfrom agricultural soilsand28.57% of those from pastures showed multi-resistant against amoxicillin, ampicillin, vancomycine and tetracycline. 62.5% of Enterobacterstrains from agricultural soils, 33.33% of pastures isolates and 35% of the mining soils isolates showed resistance to these antibiotics.87.5% of the agricultural soil Pseudomonas strains and 100% of pastures isolates showed also resistance to these antibiotics. According to the results, the resistance to streptomycin and doxycycline in three isolates was high, whereas it was the least for gentamicin. All isolates displayed resistance to lead and sensitivity to cadmium. All agricultural soil Azotobacterstrains, 57% of pasture isolates and 66.7% of mining waste isolates were multi-resistant to Pb, Hg and Ni. Abundance of Enterobacters, resistant to these metals from agricultural, pasture and mining waste samples was 45.83, 80 and 65%. 37.50 and 66.7% of Pseudomonas strains from agricultural and mining wastes showed  also resistance to those metals. The high percentage of antibiotic-resistance may be attributed to the heavy metals in soils. Resistance to antibiotics in all soils was high. But, due to the favorable conditions ofagricultural soils, it seems that dangerous antibiotic-resistant bacteria may be spreading much more faster.

Keywords

References

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Journal of Sol Biology
Volume 5, Issue 1
September 2017
Pages 67-80

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