Effect of Two Mycorrhizal Fungi Species (Glomus mosseae and G. etunicatum) on Mineral Nutrients Uptake and Minituber Production in Potato Plantlets

Document Type : Research Paper

Authors

1 PhD student of Horticultural Department, Bu- Ali sina university, Hamedan

2 Assist Professor, Horticultural Department, Bu- Ali sina university, Hamedan

3 Associate Professor, Horticultural Department, Bu- Ali sina university, Hamedan

4 Assist Professor, soil science institute, Soil Biology Department, Karaj

5 Faculty member of Agricultural and Natural Resources Research center of Hamedan

Abstract

To evaluate the effects of mycorrhizal symbiosis on nutritional absorption, acclimation of potato plantlets, and efficiency of minituber production, plantlets of two potato cultivars (Agria and Sante) derived from tissue culture were inoculated with two species of mycorrhizal fungi. Inoculation was carried out when plantlets were transferred to the greenhouse. A pot experiment was conducted using a factorial based completely randomized design with four replications. Eight weeks after inoculation, colonization percentage was assayed. In addition, mineral contents (P, Fe, Zn, and Mn) were measured. After harvesting, minitubers were separated by size and total yield was estimated. In addition, dry matter of minitubers was determined. Results showed that mycorrhizae inoculants had significant effect (p≤0.01) on colonization and all parameters. Effect of cultivar did not show significant difference in Fe and Zn, but, in other traits, it was significant. By mean comparisons, it was demonstrated that inoculation with G. etunicatum and mixed inoculants had more positive effect on colonization intensity, minituber production, and nutrient contents, compared with G. mosseae. The highest amount of P, Fe, Zn and Mn was obtained in plantlets inoculated with the mixed inoculant, which was not significantly different than plantlets inoculated with G. etunicatum, but showed significant difference with plantlets inoculated with G. mosseae in all nutrients. The highest number of minitubers (13.81 per plantlet) was observed in mixed inoculants that significantly (p≤0.05) was different than the two separated inoculants and the control treatment. In both cultivars, dry matter of minituber was increased significantly (p≤0.05) by application of mixed inoculant and in the separately inoculated mycorrhizal treatment compared with the control treatment. Overall, inoculation of potato plantlets by the two species of mycorrhiza (both separately and mixed) caused notable increase in nutrient absorption. In turn, this effect improved the biomass of the plantlets and, consequently, helped them to acclimate better and be more efficient in minituber production. 

Keywords

References

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Journal of Sol Biology
Volume 1, Issue 1
January 2013
Pages 61-69

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