Changes of Expression Pattern of Proteins and Superoxide Dismutase Isozymes of Nodules to Drought Stress in Symbiosis of Chickpea with Three Strains of Mesorhizobium ciceri

Document Type : Research Paper

Author

Assistant Professor, Biology Department, Lorestan University

10.22092/sbj.2014.128408

Abstract

In this study, the role of rhizobium strains on symbiotic effectiveness of chickpea plants under drought stress was evaluated. Chickpea (Cicer arietinum L.) plants were inoculated with three strains of Mesorhizobium ciceri (C-15, CP-31 and CP-36) and subsequently were exposed to drought stress by withholding irrigation for two and three days. Nitrogen fixation decreased in three symbiotic associations, but decline level was higher in the plants inoculated with CP-31 and CP-36 strains. Re-watering of plants under drought stress for three days led to a partial recovery of nitrogen fixation rate in C-15 strain, but there was no recovery in CP-31 and CP-36 strains. Changes of protein profiles of the nodules inoculated with the three studied strains were analyzed by using SDS-PAGE under normally irrigated condition, no irrigation for 2 and 3 days, and no irrigation for three days followed by a watering period of three days. Protein pattern in nodules infected with C-15 strain did not change in response to drought stress, but in nodules infected with CP-31and CP-36 strains, some protein bands were identified whose expression specifically decreased under drought and, did not recover after re-watering,. Electrophoresis analysis of superoxide dismutase isoenzymes from nodules infected with the studied three strains did not show significant difference under drought compared with the control. However, after application of drought stress, superoxide dismutase activity of nodules increased in symbiosis with C-15, whereas it decreased in symbiosis with CP-31 and CP-36. In conclusion, tolerance level of symbioses to drought stress could be enhanced by selection of effective rhizobium strains.

Keywords

References

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Journal of Sol Biology
Volume 1, Issue 2
February 2014
Pages 71-82

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