Potential of Nanoparticle Nanoprosil-1 as the Carrier of Phosphate Solubilizing Bacteria in Nanobiologic Inoculants Production Process

Document Type : Research Paper

Authors

1 Former MSc. Student, University of Tehran

2 Associate Professor, University of Tehran

3 Assistant Professor, University of Tehran

10.22092/sbj.2014.128409

Abstract

The purpose of this study was to determine the capability of bacteria Pseudomonas fluorescence survival as one of phosphate solubilizing bacteria on nanoprosil-1 as a bacterial carrier in nano-biologic inoculant production. The examined carrier treatments were nanoprosil-1, vermicompost, bentonite, rock phosphate and their different mixtures. After inoculation of carriers by Pseudomonas fluorescence bacteria, inoculants were incubated at 28Ċ for 15days and then preserved at 4 Ċ for 180 days. Bacteria populations were measured at times 0, 15, 30, 60, 90, 120, 150 and 180 day by CFU method. The results of 15- day incubation showed decrease of bacteria population except for vermicompost treatment. The least count (about zero) was observed in nanoprosil-1 and in the combined treatment (nanoprosil-1 + bentonite + rock phosphate) , and the highest population   of  7.77×107 CFU was measured  in the vermicompost  treatment. At the end of maintenance period,  population of the combined treatments containing nanoprosil-1 (vermicopmost+bentonite+ nanoprosil-1 and nanoprosil-1+ bentonite+ vermicompost+ rock phosphate) increased in comparison to vermicompost (2.42×107) and showed the highest populations of 6.45×107 and 4.19×107, respectively. Based on the results of this study, the application of  nanoprosil-1 alone as bacterial carrier  was not appropriate  probably because of high pH and EC and other undefined factors.  Using of  nanoprosil-1 mixed  with other carriers due to their moderating effect  can be a novel progress in nano-biologic inoculants production.

Keywords

References

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Journal of Sol Biology
Volume 1, Issue 2
February 2014
Pages 83-92

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