بررسی اثر هم‌ افزایی کاربرد قارچ‌های میکوریز آربوسکولار بر ثبیت همزیستی نیتروژن در گیاه عدس تحت شرایط تنش کم آبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد دانشگاه تهران

2 استاد دانشگاه تهران

3 دانشیار موسسه تحقیقات خاک و آب، سازمان تحقیقات،آموزش وترویج کشاورزی، کرج، ایران

چکیده

تثبیت زیستی نیتروژن در سیستم همزیستی ریزوبیا-لگوم به شدت تحت تأثیر منفی تنش کم آبی قرار می­گیرد. در سال‌های اخیر ریزسازواره­های مفید خاکزی به‌عنوان یکی از راه­کارهای کاهش اثرات تنش خشکی و افزایش تولید محصول در کشاورزی پایدار ارزیابی‌ شده‌اند. بررسی اثر هم افزایی کاربرد قارچ­های میکوریز آربوسکولار و تثبیت زیستی نیتروژن درگیاه عدس تحت شرایط تنش کم آبی آزمایشی در آرایش فاکتوریل بصورت طرح کامل تصادفی شامل دو فاکتور، تنش رطوبتی در چهار سطح تنش2/0، 4/0، 6/0 و تنش کم 8/0رطوبت قابل دسترس گیاه و فاکتور دوم نوع قارچ میکوریزی در چهار سطح Rhizophagus intraradices و Funneliformis mosseae، ترکیب این دو گونه و شاهد منفی در گلخانه­ی تحقیقاتی گروه علوم و مهندسی خاک دانشگاه تهران انجام گرفت. نتایج نشان داد در اثر تنش کم آبی، تعداد گره، وزن خشک ریشه و اندام هوایی، محتوای کلروفیل ((spad، کلنیزاسیون ریشه، نیتروژن شاخساره به طور معنی­دار کاهش یافت.تفاوت معنی­داری در وزن خشک اندام هوایی و ریشه گیاه و درصد نیتروژن اندام هوایی گیاهان تلقیح شده با میکوریز، با گیاهان غیر میکوریزی مشاهده شد. در اثر کاربرد میکوریز تعداد گره، نیتروژن اندام هوایی، وزن خشک ریشه و اندام هوایی، کلروفیل و درصد کلنیزاسیون ریشه افزایش یافت.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluating synergistic effects of arbuscular mycorrhizal fungi on symbiotic nitrogen fixation in lentil plant under water stress conditions

نویسندگان [English]

  • B. Abolfazlibehrooz 1
  • H. A. Alikhani 2
  • F. Rejali 3
چکیده [English]

Biological nitrogen fixation would be affected severely by water stress. In recent years, beneficial microorganisms were evaluated as one of the practical methods to reduce the effects of drought stress and increase agricultural production. To investigate the synergistic effects of mycorrhizal fungi on symbiotic nitrogen fixation in lentil plant under water stress conditions, a completely randomized factorial design was conducted in green house condition. The first factor had four levels of water stress (0/2, 0/4, 0/6 and 0/8 field capacity). The second factor had four microorganism types (Rhizophagus intraradices, Funneliformis mosseae, their combination and negative control). The results showed that in water stress treatments, the number of root nodules, root and shoot dry weight, chlorophyll fluorescence, root colonization percentage, total nitrogen content decreased significantly. When plants inoculated by mycorrhizal fungi, shoot N content and shoot and root dry weight of inoculated were significantly different compared to non-mycorrhizal plants. Nodule number, shoot nitrogen content, shoot and root dry weight, chlorophyll fluorescence, and root colonization percentage were increased by inoculation of mycorrhizal fungi.

کلیدواژه‌ها [English]

  • Growth index؛ mycorrhizal fungi؛ Water stress؛ Funneliformis mosseae
  • Rhizophagus intraradices

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زیست شناسی خاک
دوره 4، شماره 2
اسفند 1395
صفحه 123-134

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